Figure 5.

Mutation of TPST Affects the Expression of PINs and Auxin Biosynthesis-Related Genes.

(A) to (D) Expression pattern of the DR5:GUS and DR5:GFP reporters in the wild type (WT; [A] and [C]) and aqc1-1 ([B] and [D]) at 4 DAG.

(E) and (F) PIN4_pro:GUS expression in the wild type (E) and aqc1-1 (F) at 4 DAG. Black arrowheads indicate the stem cell niche.

(G) and (H) PIN4 expression in 4-DAG wild type (G) and aqc1-1 (H) revealed by RNA in situ hybridization with a PIN4 antisense probe. Insets represent the sense PIN4 probe.

(I) and (J) PIN4 expression in the wild type (I) and aqc1-1 (J) revealed by immunohistochemical localization assays using anti-PIN4 antibody in 4-DAG seedlings.

(K) and (L) PIN3_pro:PIN3:GFP expression in the wild type (K) and aqc1-1 (L) at 6 DAG.

(M) and (N) PIN7_pro:PIN7:GFP expression in the wild type (M) and aqc1-1 (N) at 6 DAG.

(O) Quantification of PIN3_pro:PIN3:GFP and PIN7_pro:PIN7:GFP GFP fluorescence in the wild type and aqc1-1 as shown in (K) to (N). Data shown are average and sd. Asterisks denote Student’s t test significant difference between wild-type and mutant plants (*P < 0.01).

(P) and (Q) YUCCA2_pro:GUS expression pattern in 5-DAG wild-type (P) and aqc1-1 (Q) root tips.

(R) and (S) ASB1_pro:GUS expression pattern in 5-DAG wild-type (R) and aqc1-1 (S) root tips.

(T) and (U) ASA1_pro:GUS expression pattern in 5-DAG wild-type (T) and aqc1-1 (U) root tips.

Bars = 50 μm.

(V) Comparison of free IAA levels between 5-d-old wild-type and aqc1-1 root tips. IAA was extracted from apical 5-mm root sections. Data presented are mean values of nine biological repeats with sd. Asterisk denotes Student’s t test significant difference between the wild type and mutant (*P < 0.01).

(W) Comparison of free IAA levels between 5-d-old wild-type and aqc1-1 seedlings. IAA was extracted from whole seedlings. Data presented are mean values of nine biological repeats with sd. No significant difference was detected between the wild type and mutant (Student’s t test, P = 0.16).