Skip to main content
. 2010 Nov 2;22(11):3692–3709. doi: 10.1105/tpc.110.075721

Figure 7.

Figure 7.

Overexpression of PLT2 Partially Rescues the Root Meristem Defects of aqc1-1.

(A) to (D) Lugol staining of 7-DAG 35Spro:PLT2:GR/wild type, and 35Spro:PLT2:GR/aqc1-1 roots without ([A] and [C]) or 2 d after 2 μM DEX treatment ([B] and [D]). Pink bars represent the root meristem of different plants extending from the QC to the transition zone. Bars = 50 μm.

(E) Cortical cell numbers in meristems of the indicated plants. Data shown are average and sd (n = 16 to 23).

(F) Phenotypes of 7-DAG 35Spro:PLT2:GR/wild type and 35Spro:PLT2:GR/aqc1-1 seedlings without or 2 d after 2 μM DEX treatment. Bar = 2 mm.

(G) A simplified model showing that TPST and the Tyr-sulfated RGF peptides maintain the root stem cell niche by defining the expression of the stem cell transcription factor PLTs. Auxin induces the expression of TPST and TPST shows a positive effect on the expression of PIN3/4/7, several auxin biosynthetic genes, and PLT1/2. Given that PLT1/2 positively regulates PIN3/4/7 expression (Blilou et al., 2005), reduced expression of PIN3/4/7 in the TPST loss-of-function mutant could be a result of downregulation of PLT1/2.