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. 2010 Nov 23;22(11):3589–3602. doi: 10.1105/tpc.110.074542

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© 2010 American Society of Plant Biologists

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Figure 5. — Physicochemical Analysis of the Interaction between GID1^P99A and GA₄ Using SPR.

(A) GID1^WT–GA₄ interaction. CM5 sensor chips upon which GST-GID1^WT was immobilized were injected with solutions containing 0.0625, 0.125, 0.25, 0.5, and 1 μM GA_4. RU, resonance units.

(B) GID1^P99A–GA₄ interaction. CM5 sensor chips upon which GST-GID1^P99A was immobilized were injected with solutions containing 0.0625, 0.125, 0.25, 0.5, and 1 μM GA₄.

(C) Various kinetic values; k_a (association rate), k_d (dissociation rate), and K_D (k_d/k_a, dissociation constant), of GID1^P99A–GA₄ and GID1^WT–GA₄ interactions.