Figure 8.
Some Dicot GID1b-Type Proteins Show GA-Independent Interaction with Arabidopsis GAI.
(A) Phylogenetic analysis of dicot GID1s. Protein names of poplar GID1s defined by Mauriat and Moritz (2009) are shown next to each slash. A text file alignment used in this analysis is available as Supplemental Data Set 2 online.
(B) Y2H assay using various dicot GID1s and the mutated GID1s as bait and GAI as prey in the presence or absence of GA4. In soybean GID1b-2 (1a-1-loop) and soybean GID1b-2 (1a-2-loop), the entire loop region of soybean GID1b (from S82 to K102) was replaced with the corresponding region from soybean GID1a-1 and soybean GID1a-2, respectively. β-Gal activity was determined by a liquid assay of yeast strain Y187 transformants (means ± sd; n = 3). Equal expression level of bait proteins in yeast cells was confirmed by immunoblot analysis (see Supplemental Figure 6C online).