Figure 4.

FIM5-GFP, Expressed under Control of the FIM5 Promoter, Decorates Actin Filaments in Pollen Grains and Pollen Tubes.

Representative images of complemented fim5 plants showing that FIM5-GFP decorates actin filaments in pollen grains (A) and in pollen tubes of differing lengths ([B] to [E]). About 50% of pollen tubes show accumulation of GFP signal at the subapical region, as shown in (E). The step size of optical section is 0.5 μm for pollen grains and 0.4 μm for pollen tubes. Maximal projection of confocal optical sections throughout the entire pollen grain or pollen tube was performed. See more examples in Supplemental Figure 4 online. Bars = 10 μm.