Figure 7.

qRT-PCR Analysis of Expression of Chloroplast Division-Related Genes at Different Developmental Stages of Rosette Leaves.

The relative gene expression is compared with that of the wild type, and the ratios were rescaled by setting the expression of corresponding wild-type genes to 1.0. The data were from three biological replicates and presented as means ± sd. Significant differences were analyzed using a heteroscedastic Student’s t test (*P < 0.05; **P < 0.01).

(A) Expression of chloroplast division-related genes in the kasI mutant. Stage 1, the 8th rosette leaf at 16 DAG (the length of the rosette leaf is <3 mm); stage 2, the 7th rosette leaf at 16 DAG (the length of the leaf is ~1 cm); stage 3, the 7th rosette leaf at 23 DAG (the variegated phenotype of kasI is most distinct); stage 4, the 7th rosette leaf at 28 DAG; stage 5, the 7th rosette leaf at 35 DAG (the chloroplast size of kasI is almost to the same as that of the wild type).

(B) Expression of FtsZ1, FtsZ2, and MinE genes in transgenic kasI lines with complementary expression of KASI (lines 101, 104, and 110 at 23 DAG) are recovered and similar to that of the wild type (WT).