Measurement of PSII Activity Estimated Using Chlorophyll Fluorescence during High-Light Irradiation.
(A) to (D) Relative ratio of PSII activity without lincomycin. Mature leaf discs from Col, var2-1, var2(WT), var2(267), var2(488), and var2(267 488) were exposed to low light (250 μmol photons m−2 s−1) (A), moderate light (500 μmol photons m−2 s−1) (B), high light (1000 μmol photons m−2 s−1) (C), or strong light (1500 μmol photons m−2 s−1) (D); the maximum photochemical efficiency (Fv/Fm) was measured at 0, 30, 60, 120, 180, and 240 min after irradiation (mean ± sd; data were obtained from five biological replicates).
(E) An identical experiment to that described in (C) under strong light (1500 μmol photons m−2 s−1) except plants were infiltrated with lincomycin prior to irradiation, as shown in Figure 5.