Figure 1.

Behavior of microglia and astrocytes in response to iv LPS administration. Rats were injected iv with LPS (250 µg) dissolved in 250 µl PBS, or with PBS alone. At the indicated times after injection, brains were removed and prepared for immunohistochemistry as described in Methods. Midbrain sections (30 µm in thickness) were stained with anti-Iba-1 (A) or anti-GFAP antibody (B), and expression of Iba-1 or GFAP was visualized using peroxidase-conjugated secondary antibodies. Scale bars: 200 µm in the two left columns; 20 µm in the two right columns.