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. 2010 Oct 19;42(12):823–832. doi: 10.3858/emm.2010.42.12.085

Figure 3.

Figure 3

Expression of proinflammatory mediators in the brain in response to iv LPS injection. (A) mRNA was isolated from the midbrain regions of rats at the indicated times after iv LPS injection, and the levels of mRNAs encoding IL-1β, TNF-α, and IL-6 were analyzed by Q-PCR. Values are means ± SEMs of data from 3-5 animals. *P < 0.05; **P < 0.01, compared to intact brain. (B, C) Sections were obtained from the midbrain at the indicated times after LPS or PBS injection, and stained with antibodies detecting IL-1β, CD68, or iNOS; peroxidase-conjugated secondary antibodies (B) or double-labeled antibody combinations (C) were used to visualize binding. Midbrain sections of brains from animals injected iv with LPS were obtained at the indicated times and stained with the combinations of antibodies shown. Scale bars: 50 µm (upper panels in B); 20 µm (inset in the upper panel, and the lower panels in B); and 10 µm (C).