Skip to main content
. Author manuscript; available in PMC: 2011 Jan 5.
Published in final edited form as: J Biol Chem. 2006 Apr 24;281(26):18059–18068. doi: 10.1074/jbc.M601903200

FIGURE 5. Aldosterone down-regulates AF9 expression at both mRNA and protein levels.

FIGURE 5

A, immunoblots showing that aldosterone dynamically regulated AF9 protein expression in mIMCD3 cells. mIMCD3 cells were cultured in Dulbecco's modified Eagle's medium/F-12 plus 10% charcoal-stripped fetal bovine serum for at least 50 h, then treated with vehicle (ethanol, –) or 1 μM aldosterone (+) for the indicated times. The whole cell lysates were analyzed by immunoblot assay with the antibodies specific for AF9 antibody or α-tubulin. B, real-time RT-qPCR demonstrating that aldosterone decreased AF9 mRNA expression. Total RNAs from the mIMCD3 cells treated as in A for 1.5 h were analyzed by real-time RT-qPCR for expression of AF9 as described in the legend to Fig. 3A. n = 3.