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. 2001 Feb 27;98(5):2413–2418. doi: 10.1073/pnas.051634798

Figure 4.

Figure 4

Effect of BFA and phospholipids on [35S]GTPγS binding to ARF 6. (A) Samples (25 pmol) of ARF6 plus 2.5 pmol of ARF-GEP100 (■) or yeast ARF2 (25 pmol) plus 2.5 pmol of yeast Sec7 (●) were incubated for 4 h at 4°C with 4 μM [35S]GTPγS and the indicated amounts of BFA. (B) Samples (25 pmol) of ARF6 or ARF1 were incubated without or with 2.5 pmol of ARF-GEP100 (p100) for 20 min at 30°C with 4 μM [35S]GTPγS and the indicated amount of phospholipid (PS, PIP2, PIP3). Data are means ± SEM of values from triplicate assays. Experiments were repeated twice with similar results.