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Effect of calcium and calmodulin on [35S]GTPγS. Samples (25 pmol) of ARF6 were incubated for 20 min at 30°C with 2.5 pmol of ARF-GEP100 and 4 μM [35S]GTPγS without (□) or with (■) 100 nM calmodulin and the indicated concentration of CaCl2(Ca2+) (Left), or without (○) or without (●) 100 μM CaCl2 and the indicated concentration of CaM (Right). Data are means ± SEM of values from triplicate assays. IQ motif sequence above is compared with sequence of ARF-GEP100 (p100).
