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Subcellular distribution of ARF-GEP100 and ARF6. T98G cells were homogenized in buffer with 1 mM MgCl2 (Mg2+) or 1 mM EDTA, and postnuclear supernatant, crude membrane (M), and cytosol (C) fractions were prepared. Samples were subjected to SDS/PAGE and immunoblotting with anti-ARF-GEP100 (p100) and anti-ARF6 antibodies. Experiment was replicated three times.
