Table 1.
Ratio of ascorbate between organelles
| Ratio of ascorbate between organelles | ||||||
|---|---|---|---|---|---|---|
| Mitochondria | Chloroplasts | Nuclei | Peroxisomes | Cytosol | Vacuole | |
| Arabidopsis | ||||||
| Conventional fixation | 12.2 | 12.8 | 19.4 | 27.1 | 25.8 | 2.7 |
| Microwave fixation | 12.5 | 13.9 | 19 | 25.9 | 24.8 | 3.9 |
| Edge of the sample | 11.4 | 13.7 | 20.5 | 25.2 | 25.8 | 3.4 |
| High-pressure freezing | 12.9 | 10.9 | 22.2 | 25.4 | 24 | 4.6 |
| Nicotiana | ||||||
| Conventional fixation | 11.9 | 10.7 | 31.9 | 10 | 31.4 | 4.1 |
| Microwave fixation | 11.3 | 12.2 | 30.3 | 10.2 | 30.4 | 5.6 |
| Edge of the sample | 11.2 | 10.5 | 33.8 | 9.4 | 31.1 | 4 |
| High-pressure freezing | 10.7 | 11.3 | 31.5 | 11.3 | 29.6 | 5.6 |
Ratio (in %) of the distribution of gold particles bound to ascorbate (total amount of gold particles per μm2) between cell compartments of plants prepared with conventional and microwave fixation and at the edge of the sample after conventional fixation and after high-pressure freezing and freeze substitution