Skip to main content
. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Mol Immunol. 2010 Dec 15;48(4):683–690. doi: 10.1016/j.molimm.2010.11.014

Fig. 1.

Fig. 1

Serum factor H binding to mid-logarithmic S. aureus. S. aureus were incubated with 10% normal human serum in GVBS++ for the time indicated at 37°C. Cell-wall proteins were solubilized from washed bacteria and analyzed via anti-fH Western blot: (A) Representative Western blot showing strain Newman (296), Reynolds and the clinical isolates S10 and S6; (B) Strain Reynolds and a clinical strain (R7). (C) R7 was incubated with 10% NHS in GVBS for 30 mins at 37°C, washed, then bound proteins eluted with 0.1M glycine, pH 2.5. (D) Bacteria were incubated with various concentrations of NHS in GVBS++ for 30 mins at 37°C. Bound fH was stripped from washed bacteria with 2% SDS at 95°C; fH binding was measured via ELISA. Data represent the mean of three independent experiments ± SEM. Abbreviations: factor H (fH), Factor H-like protein 1 (FHL-1), Factor H related protein 1 (FHR 1).