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90%:10% (NIKSGFP : primary keratinocytes) chimeric skin substitute evaluated for expression of E-cadherin (A, E) keratin 1 (B, F) transglutaminase-1 (C, G) and filaggrin (D, H). GFP fluorescence (green) permitted visualization of tissue chimerism, whereas Hoechst 33258 (blue) identified nuclei localization. A white dashed line denotes the dermal / epidermal boundary. Scale bar represents 100 μm.
