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. 2010 Dec 9;1(12):e106. doi: 10.1038/cddis.2010.84

Figure 5.

Figure 5

Knockdown of PI4KIIα or PI4KIIIβ in MDA-MB-231 cells. MDA-MB-231 cells were mock transfected or transfected with PI4KIIα or PI4KIIIβ siRNA. (a) Time course of PI4K knockdown. Cells were analyzed by immunoblotting against PI4KIIα, PI4KIIIβ or GAPDH at 0, 24, 48 and 72 h after transfection. Representative blot from five experiments are shown. (b) Western blot of dilution series of non-transfected COS-7 or MDA-MB-231 cell lysates showing relative levels of PI4Ks compared with GAPDH. Blot shown is representative of two independent experiments. Note that differences in expression are most evident at 1 : 4 dilution