Table 1.
Summary of cGMP responses of cygnet-2 transfected RFL cells
| Stimulus | Cells tested | Responsive cells | Mean lag time (sec) | % Increase in ratio | Calibration maximum |
|---|---|---|---|---|---|
| 0.1 mM NONO | 25 | 17 | 126 ± 116 | 19.1 | 20.2 |
| NONO with 0.6 mM IBMX | 21 | 9 | 264 ± 280 | 19.1 | 20.3 |
| NONO before IBMX | 7 | 1 | 500 | 24 | 30.3 |
| NONO after IBMX | 6 | 6 | 253 ± 321 | 18 | 20.1 |
| NONO (second application) | 6 | 6 | 170 ± 82 | 17.5 | 24.3 |
| 1 μM CNP | 13 | 13 | 71 ± 54 | 16.9 | 21.3 |
| CNP (second application) | 16 | 15 | 118 ± 116 | 17.5 | 23 |
| IBMX before any other stimulus | 8 | 0 | NA | NA | |
| 50–100 μM 8-CPT-cGMP | 14 | 14 | 1910 ± 1116 | 24.8 | 24.8 |
Responsive cells are those with a >5% change in emission ratio. Mean lag time is the time from stimulus addition to the beginning of a clear response. % increase in ratio is the mean amplitude of the response. Calibration maximum is the % increase in ratio produced by saturating stimulation such as 8-CPT-cGMP. NA, not applicable.