Skip to main content
. 2001 Feb 20;98(5):2443–2448. doi: 10.1073/pnas.041493198

Figure 3.

Figure 3

Detection of PPARγ in BM and RAW264.7 cells. (A) Regulation of PPARγ1 mRNA by GM-CSF, M-CSF, and IL-4 in BMs but not in RAW264.7 cells. BMs and RAW264.7 cells were cultured in the absence or presence of one of the following cytokines for 24 h: vehicle (lane 1); mGM-CSF (10 ng/ml, lane 2), M-CSF (10 ng/ml, lane 3), or IL-4 (10 ng/ml, lane 4). Total RNA was isolated, treated with DNase, and subjected to reverse transcription–PCR analysis. (B) Detection of PPARγ1 protein. BMs were incubated untreated (lane 1) or were treated with 10 ng/ml M-CSF for 48 h (lane 2) or 72 h (lane 3) or with 100 ng/ml M-CSF for 48 h (lane 4) or 72 h (lane 5), and nuclear extracts (75 μg) were evaluated by Western analysis.