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. 2001 Feb 20;98(5):2443–2448. doi: 10.1073/pnas.041493198

Figure 7.

Figure 7

12/15-LO and COX-1/COX-2 are not required for IL-4 action. (A) BMs were isolated from mice heterozygous (+/−) or homozygous (−/−) for the 12/15-lipoxygenase null allele, plated in triplicate (1 × 105 cells per well), and induced with M-CSF/RANKL in the absence or presence of increasing amounts of IL-4 (0.1, 1, and 10 ng/ml). Multinucleated (more than three nuclei), TRAP-positive osteoclasts were quantitated after 10 days. (B) BMs from normal mice were treated for 10 days with M-CSF/RANKL, IL-4 (0.5 ng/ml), or ibuprofen (10 or 100 μM) as indicated. Mean ± SE, n = 3.