Table 1.

Frequency of germ-line chimerism

Group of injected embryos*	Survivors†	Germ-line chimeric‡	F1 transmission§	F2 frequency¶
1	49	1	19/342	198/267
		1	1/82	—
2	16	0	0	—
3	26	1	1/81	—
		1	8/97	93/126
4	8	0	0	—
Total	99	4	29	—

^*Each group of embryos was injected with cultured cells on different days. 

^† Injected embryos that survived to sexual maturity. 

^‡ Survivors were bred with GASSI fish and germ-line chimeras were identified by the production of neo⁺ pigmented F1 fish. 

^§ Number of neo⁺ pigmented F1 fish produced by each germ-line chimera/total number of embryos produced. 

^¶ Number of neo⁺ pigmented F2 fish produced by breeding positive sibling fish from the F1 generation/total number of embryos produced.