Skip to main content
. Author manuscript; available in PMC: 2011 Jan 5.
Published in final edited form as: Dev Dyn. 2009 Sep;238(9):2292–2308. doi: 10.1002/dvdy.22036

Figure 6. Reduced accumulation of pERK in transition zone of lenses from Dlg10 and Dlg39 mice.

Figure 6

Longitudinally oriented, paraffin embedded eye sections from P2 control (A,D,G,J), Dlg10 (B,E,H,K) and Dlg39 (C,F,I,L) mice were subjected to immunoflourescence analysis for three markers of fiber cell differentiation. A-C: Merged images of control (A), Dlg10 (B) and Dlg39 (C) lenses stained with To-PRO3 (blue nuclei) and an anti-pERK antibody (red). D-F: Unmerged images corresponding to A-C show anti-pERK staining (red) only. White boxes show the patchy and reduced accumulation of pERK staining in the transition zone in Dlg10 (E) and Dlg39 (F) mice. G-H: Sections from control (G), Dlg10 (H) and Dlg39 (I) mice were immunostained with anti-MIP26 antibodies. The pattern of MIP26 immunostaining was not altered in lenses of Dlg10 or Dlg39 mice as compared to controls. J-L: Sections of control (J), Dlg10 (K) and Dlg39 (L) mice were immunostained with anti-β crystallin antibodies. The pattern of β-crystallin immunostaining was not altered in lenses of Dlg10 or Dlg39 mice as compared to controls. e=epithelium, f=fibers. Scale bar=50μm.