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. Author manuscript; available in PMC: 2011 Jan 5.
Published in final edited form as: Dev Dyn. 2009 Sep;238(9):2292–2308. doi: 10.1002/dvdy.22036

Table1.

PCR Primer Combinations

Combination Primer
Names
Sequence Expected Size Identifies
1 Dlg5′
Forward
5′ CAT CAT GGT TGA AGT GCT
CTG GGC 3′
Wt=540bp
Floxed=753bp
Dlg-1 Wt
and Floxed
Alleles
DlgI8R
Reverse
5′AAA TGT GGC CTG AGG ATC
TAC CTC CG 3′
2 Dlg5′
Forward
5′ CAT CAT GGT TGA AGT GCT
CTG GGC 3′
Wt=1058bp
Floxed=1273bp
CKO=380bp
Wt, CKO,
and Floxed
allele
Dlg3′
Reverse
5′ GGA AGG AAA CTC ACG GAT
GGT CC 3′

3 Pr4 5′ GCA TTC CAG CTG CTG ACG
GT 3′
Cre Band= 577bp Cre
transgene
CreAS 5′ CAG CCC GGA CCG ACG ATG
AAG 3′