Table1.
Combination | Primer Names |
Sequence | Expected Size | Identifies |
---|---|---|---|---|
1 | Dlg5′ Forward |
5′ CAT CAT GGT TGA AGT GCT CTG GGC 3′ |
Wt=540bp Floxed=753bp |
Dlg-1 Wt and Floxed Alleles |
DlgI8R Reverse |
5′AAA TGT GGC CTG AGG ATC TAC CTC CG 3′ |
|||
2 | Dlg5′ Forward |
5′ CAT CAT GGT TGA AGT GCT CTG GGC 3′ |
Wt=1058bp Floxed=1273bp CKO=380bp |
Wt, CKO, and Floxed allele |
Dlg3′ Reverse |
5′ GGA AGG AAA CTC ACG GAT GGT CC 3′ |
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| ||||
3 | Pr4 | 5′ GCA TTC CAG CTG CTG ACG GT 3′ |
Cre Band= 577bp | Cre transgene |
CreAS | 5′ CAG CCC GGA CCG ACG ATG AAG 3′ |