Table 2.

Characterization of GTEV/IGTE clones generated by electroporation of GTEV49 and GTEV85 ES cells with pIGTE2-hAP

GTEV/IGTE clone	Dosage of hAP in cell lysates			Expression of hAP‖ in 8-day-old embryoid bodies (in situ histochemistry)	Expression of hAP** in chimeras at E10 (in situ histochemistry)
	−OHT (OD)	+OHT‖ (OD)	Induction +OHT−OHT
49–30	1539	769	—	ND	ND
49–33*	15	96	7	+++	ND
49–110*	4†	157‡	39	+++§	+++¶
49–113	62	177	3	ND	ND
49–126	1280	1099	—	ND	ND
49–134	104	57	—	ND	ND
49–143	910	999	1.1	ND	ND
49–146*	0	150	∞	—	ND
49–160	0	21	∞	ND	ND
49–162	24	31	1.3	ND	ND
49–181	29	15	—	ND	ND
49–184*	15	271	18	+++	ND
49–185	0	32	∞	ND	ND
85–1	402	263	—	ND	ND
85–12	24	93	3.9	ND	ND
85–39	63	87	1.4	ND	ND
85–47	108	113	1	ND	ND
85–101	5	26	5.2	ND	ND
85–149*	0	332	∞	+++	++++
85–150	21	44	2.1	ND	ND

+++/++++, strong/very strong induction of hAP expression; −, no induction of hAP expression; ND, not determined; OD, optic density at 420 nM. 

^*Indicates clones selected for strong induction of hAP expression in response to 4′OHT treatment. 

^† Shown in Fig. 3B. 

^‡ Shown in Fig. 3C. 

^§ Shown in Fig. 3E. 

^¶ Shown in Fig. 5D. 

^‖ +4′OHT (1 μM, 48 hours). 

^**+4′OHT (1 mg i.p.).