Figure 4.
Expression of Gal-1 and adhesion molecule (β2-integrin and L-selectin) in transmigrated neutrophils (Nφs) of mesenteric tissue. Nφs showed a significant proportion of Gal-1 (arrows; 15 nm colloidal gold) and β2-integrin (arrowheads; 10 nm colloidal gold) co-localization in the plasma membrane and cytoplasm (A) that was poorly observed for L-selectin (B; arrowheads; 10 nm colloidal gold) at4h of peritonitis. Nucleus (N). Pretreatment with hrGal-1 induced diminished β2-integrin (C) and augmented L-selectin expression (D). (E) Absence of gold labeling in control section of reaction. Scale bars, 0.5μm. Density of β2-integrin (F) and L-selectin (G) immunogold particles in intravascular (Nφin) and transmigrated Nφs (Nφout) at 4 h post-zymosan administration in mice pretreated or not with hrGal-1. Data are mean ± SEM of 10 distinct cells for each group analyzed in the mesenteric tissue samples of 4 mice. *P < 0.01 versus correspondent Nφin and Nφout values.