Fig. 5.

Expression analysis of different genes in the five ERAD deletion strains on the scGlaGus background, scGlaGus parental strain, mcGlaGus and N402. Total RNA was extracted from mycelia grown for 24 h at 30°C in CM. RNA (5 μg) was separated by agarose gel electrophoresis, blotted and hybridized with ³²P-labeled probes specific for the genes indicated. 18S rRNA was used as loading control