Table 1.
Strains used in this study
| Strain | Genotype | Description | Reference |
|---|---|---|---|
| N402 | cspA1 derivative of ATCC9029 | – | Bos et al. 1988 |
| MA70.15 | ΔkusA::amdS + in AB4.1 pyrG - | – | Meyer et al. 2007 |
| MA78.6 | ΔkusA::amdS + in N402 | – | Carvalho et al. 2010 |
| NC5 | FAA-resistant derivative from MA78.6ΔkusA, amdS -) | – | Carvalho et al. 2010 |
| MA97.2 | ΔderA::amdS in NC5 | – | This study |
| MA98.1 | ΔdoaA::amdS in NC5 | – | This study |
| MA94.3 | ΔhrdC::amdS in NC5 | – | This study |
| MA95.9 | ΔmifA::amdS in NC5 | – | This study |
| MA96.6 | ΔmnsA::amdS in NC5 | – | This study |
| MV3.2 | pBB19-3pyrG* in MA70.15 (ΔkusA::amdS + ) | pGpdA-Gla514-Gus-pyrG* | This study |
| MA99.3 | FAA-resistant derivative from MV3.2 | pGpdA-Gla514-Gus-pyrG* | This study |
| MA110.1 | ΔderA::amdS in MA99.3 | pGpdA-Gla514-Gus-pyrG* | This study |
| MA111.3 | ΔdoaA::amdS in MA99.3 | pGpdA-Gla514-Gus-pyrG* | This study |
| MA112.10 | ΔhrdC::amdS in MA99.3 | pGpdA-Gla514-Gus-pyrG* | This study |
| MA113.2 | ΔmifA::amdS in MA99.3 | pGpdA-Gla514-Gus-pyrG* | This study |
| MA114.7 | ΔmnsA::amdS in MA99.3 | pGpdA-Gla514-Gus-pyrG* | This study |
| MA115.1 | ΔderA FAA-resistant derivative from MA97.2 | – | This study |
| MA116.2 | ΔhrdC FAA-resistant derivative from MA94.3 | – | This study |
| MA117.1 | ΔmifA FAA-resistant derivative from MA95.9 | – | This study |
| MA118.2 | ΔmnsA FAA-resistant derivative from MA96.6 | – | This study |
| MA119.1 | ΔhrdC, ΔderA::amdS (ΔderA::amdS in MA116.2 | – | This study |
| MA120.1 | ΔhrdC, ΔdoaA::amdS (ΔdoaA::amdS in MA116.2 | – | This study |
| MA122.4 | ΔhrdC, ΔmnsA::amdS (ΔmnsA::amdS in MA116.2 | – | This study |
| MA123.7 | ΔmifA, ΔderA::amdS (ΔderA::amdS in MA117.1 | – | This study |
| MA124.2 | ΔmifA, ΔdoaA::amdS (ΔdoaA::amdS in MA117.1 | – | This study |
| MA125.1 | ΔmifA, ΔhrdC::amdS (ΔhrdC::amdS in MA117.1 | – | This study |
| MA127.3 | ΔmnsA, ΔderA::amdS (ΔderA::amdS in MA118.2 | – | This study |
| MA128.1 | ΔmnsA, ΔdoaA::amdS (ΔderA::amdS in MA118.2 | – | This study |
| MA130.3 | ΔmnsA, ΔmifA::amdS (ΔmifA::amdS in MA118.2 | – | This study |
| MA131.1 | ΔderA, ΔdoaA::amdS (ΔdoaA::amdS in MA115.1 | – | This study |
| AB4-1dglaA36#3 | [pBB19-3]#3 multicopy transformant | Multicopy pGpdA-Gla514-Gus | Punt et al. 1994, 1998 |
| MA134.64 | ΔkusA::amdS + in AB4-1dglaA36#3 (Δku70, amdS -) | Multicopy pGpdA-Gla514-Gus | This study |
| MA135.3 | FAA-resistant derivative from MA134.64 | Multicopy pGpdA-Gla514-Gus | This study |
| MA136.18 | ΔderA::amdS in MA135.3 | Multicopy pGpdA-Gla514-Gus | This study |
| MA137.2 | ΔdoaA::amdS in MA135.3 | Multicopy pGpdA-Gla514-Gus | This study |
| MA139.6 | ΔmifA::amdS in MA135.3 | Multicopy pGpdA-Gla514-Gus | This study |
| MA140.8 | ΔmnsA::amdS in MA135.3 | Multicopy pGpdA-Gla514-Gus | This study |
| AB1.13#72 | [phIL6-3A]#72pAN7-1 | IL6 (PgpdA) | Broekhuijsen et al. 1993 |
| AB1.13#54 | [pAN56-3hIL6]#54pAN7-1 | GLA::IL6 (PgpdA) | Punt et al. 1998 |
| AB1.13#38 | [pAN56-4hIL6]#38pAN7-1 | GLA::kex::IL6 (PgpdA) | Punt et al. 1998 |
| D15 | [pGpdA-GlaA::tPA]#25 | GLA::kex::tPA (PgpdA) | Wiebe et al. 2001 |
| MGG029#25 | [pGlaA-MNP1.i]#25 | MnP1 from Phanerochaete chrysosporium | Conesa et al. 2000 |
| MGG029#13 | [pGlaA-GlaA::MNP1]#13 | Mnp1 from P. chrysosporium expressed as GlaA fusion protein | Conesa et al. 2000 |
| B36 | [pAB6-10]#36 | Contain over 80 copies of the Glucoamylase gene | Verdoes et al. 1993 |
| AR1.1 | [pPgla-Gla514::GFP] | Glucoamylase-GFP | Gordon et al. 2000 |
| XW2.2.1 | [pPgla-Gla514::GFP-HDEL] | Glucoamylase-GFP fusion with ER targeting sequence | Gordon et al. 2000 |
| MA23.1.1 | [pPgpd-CPY31::GFP] | CpyA- GFP fusion expressed from gpdA promoter | Weenink and Ram, unpublished |
| NW5.1 | [pPgpd-CwpA::GFP] | CwpA-GFP from A. niger | Damveld and Ram, unpublished |
| XW5.2 | [pPgla-Gla514::POX2] | Laccase from Pleurotus ostreatus | Weenink et al. 2006 |
| XW6.1 | [pPgla-Gla514] | – | Weenink et al. 2006 |
IL6 interleukin 6, tPA tissue plasminogen activator, MnP1 manganese peroxide, Cwp cell wall protein, Cpy carboxypeptidase Y