Table 2.
Primers used throughout this study: restriction enzymes added are underlined
| Primer name | Sequence (5′ to 3′) | Amplification of | Restriction enzyme |
|---|---|---|---|
| pDER9Eco | gcgaattctgcaccccactggggcatttactgc | derA 3′ flank | EcoRI |
| pDER10Hin | gcaagctttaatcccgcacaagaagatacc | derA 3′ flank | HindIII |
| pFDERMB | gcaacgcgttgcaaaggatcctccgcgtaatcgctc | derA 3″ flank | BamHI |
| pRDERKpn | tcggtacctcggatgaggtcagagcatgctttaatc | derA 3″ flank | KpnI |
| pDER3Not | tggcggccgcggtacgcacgctgaacgtcg | derA 5′ flank | NotI |
| pDER4Bam | ggggatccttgatgggtagtagagttgcga | derA 5′ flank | BamHI |
| pdoaHinb | ctgatcgctaagcttttgcaagagctgaaccaacacgtc | doaA 3′ flank | HindIII |
| pdoaAsc | gcaaggcgcgcctacgtagagaatgaaggtcaaagtg | doaA 3′ flank | AscI |
| pdoaEco | cgtagaagattgttgaattcctgaacaatggc | doaA 5′ flank | EcoRI |
| pdoaHina | agaagcttagatcttgaactcaggcatacatagaccag | doaA 5′ flank | HindIII |
| pdoaNot | cgatagtagcggccgcaatgtgaagtgacgataaaggtg | doa 5″ flank | NotI |
| pdoaMlu | catacgcgtggccctccaaagaagcggagatcttgaactc | doaA 5″ flank | MluI |
| pHRD7Not | ttgcggccgcagcctgcaggtcgatccccttc | hrdC 5′ flank | NotI |
| pHRD8Mlu | tacgcgtcggaaggcttcttgggcgtaatg | hrdC 5′ flank | MluI |
| phrdhin | caagctttgctgcggaatgcagcgctggctcttatc | hrdC 3′ flank | HindIII |
| phrdasc | gggcgcgcccttgatatgcaatgggaatggattgtg | hrdC 3′ flank | AscI |
| phrdMlu | gacgcgttttgctgcggaatgcagcgctggctcttatc | hrdC 3″ flank | MluI |
| phrdKpn | catggtacccgtcttcgcggtggtcgtcaaggcg | hrdC 3″ flank | KpnI |
| pmifNot | cacgcggccgctgatcacggaatcggatcaaccgaggaagc | mifA 5′ flank | NotI |
| pmifXmaa | ggcccggggttacctgaagctccccgcggcattggagcag | mifA 5′ flank | XmaI |
| pmifEco | ccgaattccgcaccagggcaggctcctctgtaccttctc | mifA 3′ flank | EcoRI |
| pmifAsc | aaggcgcgccgcagtagatatatgttgcgctaatagactaag | mifA 3′ flank | AscI |
| pmifXmab | cacccgggcaggctcctctgtaccttctctcgtcaccaaac | mifA 3″ flank | XmaI |
| pmifKpn | aaggtacccttgcccagttgactgcgtgccaggtggtgc | mifA 3″ flank | KpnI |
| pmnsNot | tagcggccgcccacccccaatctacttatgctcatataatg | mnsA 5′ flank | NotI |
| pmnsXmaa | gcggtccccggggaggggattgttcagggagttggag | mnsA 5′ flank | XmaI |
| pmnsEco | caccgaattcaatgtcgacgaccctcgcgtcatggaaacagac | mnsA 3′ flank | EcoRI |
| pmnsAsc | gcggcgcgccacgacgtgtatatataacgaggaaacg | mnsA 3′ flank | AscI |
| pmnsXmab | cacccgggatcacctacttcaatgtcgacgaccctcgcg | mnsA 3″ flank | XmaI |
| pmnsKpn | gcggtaccgcctccgtattgaatacatggtcttcg | mnsA 3″ flank | KpnI |
| pNC43 | ggcgcgcctcggtcgctcactgttcct | pyrG* | AscI |
| pNC44 | ggcgcgccgacggagtagccgagagcaa | pyrG* | AscI |