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. 2011 Jan;11(1):152–155. doi: 10.1111/j.1600-6143.2010.03333.x

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© 2011 American Society of Transplantation and the American Society of Transplant Surgeons

Re-use of this article is permitted in accordance with the Creative Commons Deed, Attribution 2.5, which does not permit commercial exploitation.

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Renal transplant biopsy. (A) Light microscopy showing a normal glomerulus (periodic acid-Schiff). (B) Immunoperoxidase staining showing granular capillary wall and mesangial C3 deposition. C3 was detected using a polyclonal rabbit anti-human C3c antibody (Dako Ltd., Ely, United Kingdom). (C) Electron micrograph showing subendothelial deposits (denoted by arrows) with new basement membrane beneath. (D) Glomerular C5b-9 by indirect immunofluorescence. C5b-9 was detected using a monoclonal mouse anti-human C5b-9 antibody (DakoCytomation Ltd., Ely, United Kingdom) and a fluorescein isothiocyanate-labeled goat anti-mouse IgG (Sigma-Aldrich Company Ltd., Dorset, United Kingdom).