Table 2.

Linear range

Gene	Linear Range	Quadratic p-value	Cubic p-value	Maximum Absolute Deviation from Linearity (CT)
ATP5E	2 -10 to 25 ng	< 0.01	< 0.01	0.44
BGN	2 -9 to 25 ng	< 0.01	< 0.01	0.17
MYC	2 -10 to 25 ng	< 0.01	0.11	0.86
FAP	2 -7 to 25 ng	< 0.01	< 0.01	0.74
GADD45B	2 -7 to 25 ng	< 0.01	0.01	0.38
GPX1	2 -10 to 25 ng	< 0.01	0.04	0.30
INHBA	2 -7 to 25 ng	< 0.01	< 0.01	0.99
MK167	2 -7 to 25 ng	< 0.01	< 0.01	0.17
MYBL2	2 -6 to 25 ng	< 0.01	0.81	0.43
PGK1	2 -8 to 25 ng	< 0.01	< 0.01	0.56
UBB	2 -10 to 25 ng	< 0.01	0.08	0.36
VDAC2	2 -10 to 25 ng	< 0.01	< 0.01	0.23

Using the aggregated, non-normalized C_T values from the 17-point serial dilution, the linearity of C_T value as a function of RNA concentration was evaluated for each gene. All genes demonstrated a maximum deviation from linearity less than 1 C_T over at least an 11 log₂ concentration range (2^-6 to 2⁵).