Table 1.

Oligonucleotide primers and probes for detection of S. pneumoniae, H. influenzae and N. meningitidis.

	Sequence (5' to 3')a	Positions in target gene
S. pneumoniae
Spn9802 F	5'-A GTC GTT CCA AGG TAA CAA GTC T-3'	3370-3392
Spn9802 R	5'-AC CAA CTC GAC CAC CTC TTT-3'	3525-3506
Spn9802 FAM	5'-FAM-aTc AGa TTg CTg ATa AAa CgA-BHQ1-'3
H. influenzae
Hi P6 F	5'-CCA GCT GCT AAA GTA TTA GTA GAA G-3'	302-326
Hi P6 R	5'-TTC ACC GTA AGA TAC TGT GCC-3'	477-457
Hi P6 JOE	5'-JOE- CAg ATg CAg TTg AAg GTt Att tAG-BHQ1-'3
N. meningitidis
ctrA F	5'-GCTGCGGTAGGTGGTTCAA-3'	617-635
ctrA R	5'-TTGTCGCGGATTTGCAACTA-3'	727-708
ctrA ROX	5'-ROX-CATTGCCACGTGTCAGCTGCACAT- BHQ1-'3

^a Positions with lower case letters indicate locked nucleic acid [27].