Table 1.
Effect of purified AI-2 on encystment and germination of P. nicotianae zoospores after overnight incubation at 23°C
| Conc. of AI-2 (μM) |
No. of cysts | No. of germinating cysts |
No. of empty cells |
No. of lysed zoosporesa |
||||
|---|---|---|---|---|---|---|---|---|
| Mb | Stdb | M | Std | M | Std | M | Std | |
| 0 | 5 | 0.3 | 12 | 2.3 | 39 | 1.0 | 1 | 3.8 |
| 0.01 | 10 | 0.3 | 7 | 0.5 | 22 | 1.3 | 17 | 1.0 |
| 0.1 | 5 | 0.5 | 4 | 0.8 | 22 | 0.8 | 25 | 0.5 |
| 1 | 2 | 0.3 | 0 | 0.0 | 21 | 1.8 | 33 | 2.0 |
| 10 | 11 | 0.5 | 0 | 0.0 | 22 | 2.1 | 19 | 2.5 |
| 100 | 20 | 1.0 | 0 | 0.0 | 0 | 0.0 | 36 | 1.0 |
| 1000 | 14 | 1.3 | 0 | 0.0 | 0 | 0.0 | 42 | 1.3 |
a Difference between the total number of zoospores (56 ± 4) in SDW and those countable in AI-2 at each concentration.
b M is the mean from 12 replicate fields (at 100×) of three assays. Std is the standard deviation.