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. 2010 Dec 13;5:56. doi: 10.1186/1750-1326-5-56

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Copyright ©2010 Jiang et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<url>http://creativecommons.org/licenses/by/2.0</url>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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SB-treated cultures accumulate α-Syn of different solubility and sizes. 3D5 cells were treated with 10 mM SB for 36 hs after 10 ds of differentiation and induced α-Syn expression. Cultures without the drug treatment were used as controls (Con). Total cell lysates (TL) were fractionated to obtain buffer-soluble (SN1), buffer-insoluble and salt plus sarkosyl-soluble (SN2) as well as sarkosyl-insoluble (SKI) fractions. These samples were evaluated by immunoblotting using TL:SN1:SN2:SKI at 1:1:3.75:9.37 loading and antibodies to α-Syn. GAPDH was used as loading control. Asterisk marks monomeric α-Syn.