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. 2011 Jan 5;6(1):e15609. doi: 10.1371/journal.pone.0015609

Figure 1. Genomic organization of SeMV and Y2H assay for interacting partner of MP.

Figure 1

(A) A schematic representation of the genomic organization of SeMV, representing all the proteins encoded. ORF 1 codes for MP, ORF 2a for poly-protein 2a, ORF 2b for RdRp which is translated by a frame shift mechanism. ORF 3 codes for CP (B) Y2H interaction between MP and all the proteins encoded by SeMV. –Leu –Trp –His plate streaked with AH109 cells transformed with bait and pray plasmids (as shown in the pie diagram). Growth on the plate represents activation of the reporter gene by interaction between the proteins.