Table 2.

Sequences and exon locations of qPCR primers, amplicon lengths, PCR efficiencies and correlation coefficients

Gene Symbol	Reference sequence	Forward primer	location	Reverse primer	location	Amplicon length (bp)	PCR efficiency (%)	R2
GAPDH	NM_002046	TGCACCACCAACTGCTTA C	Ex7	GGCATGGACTGTGGTCATGAG	Ex7-8	87	109	0,99
SDHA	NM_004168	TGGGAACAAGAGGGCATCTG	Ex2	CCACCACTGCATCAAATTCATG	Ex3	86	104	0,9914
HPRT	NM_000194	TGACACTGGCAAAACAATGCA	Ex5-6	GGTCCTTTTCACCAGCAAGCT	Ex6	94	110	0,9985
IL8	NM_000584	GAATGGGTTTGCTAGAATGTGATA	Ex4	CAGACTAGGGTTGCCAGATTTAAC	Ex4	129	99	0,9995
TNF	NM_000594	CCTGCCCCAATCCCTTTATT	Ex4	CCCTAAGCCCCCAATTCTCT	Ex4	80	88	0,9991
IL17A	NM_002190	CCATAGTGAAGGCAGGAATC	Ex3	CGGTTATGGATGTTCAGGTT	Ex3	108	98	0,998
IL17F	NM_052872	AGCGCAACATGACAGTGAAG	Ex1	GTGTAATTCCAGGGGGAGGT	Ex2	105	89	0,993
IL21	NM_021803	ACTTGGTCCCTGAATTTCTGC	Ex1-2	TTTGTGGAAGGTGGTTTCCTC	Ex3	169	92	0,9891
IL22	NM_020525	TTCCAGCAGCCCTATATCACC	Ex1	GCTCACTCATACTGACTCCGTG	Ex2-3	125	102	0,9937
IL26	NM_018402	ATCAAAGCAGCATGGCTCAAA	Ex1	GCAGTTGACCAAAAACGTCTTCC	Ex3	154	104	0,9894
IL1B	NM_000576	CACGATGCACCTGTACGATCA	Ex5	GTTGCTCCATATCCTGTCCCT	Ex5	121	90	0,9991
IL6	NM_000600	ATTCTGCGCAGCTTTAAGGA	Ex5	AACAACAATCTGAGGTGCCC	Ex5	119	104	0,9956
TGFB1	NM_0006600	GGCCAGATCCTGTCCAAGC	Ex1	GTGGGTTTCCACCATTAGCAC	Ex1	201	93	0,9872
IL23A	NM_016584	TTCTGCTTGCAAAGGATCCA	Ex3	AATATCCGATCCTAGCAGCTTCTC	Ex3	64	106	0,9957
STAT3	NM_139276	GATCCAGTCCGTGGAACCAT	Ex21	ATAGCCCATGATGATTTCAGCAA	Ex21	74	106	1
CCR6	NM_031409	GTGCAAGTTGCTAAAAGGCATC	Ex3	CGAATGACTTAGTCGCCTGT	Ex3	112	89	1
CCL20	NM_004591	TGCTGTACCAAGAGTTTGCTC	Ex1	CGCACACAGACAACTTTTTCTTT	Ex3	220	98	0,9914
RORC	NM_005060	GTAACGCGGCCTACTCCTG	Ex4	GTCTTGACCACTGGTTCCTGT	Ex5	227	102	0,9944
RORC (RORC1)	NM_005060	GCAAAGAAGACCCACACCTC	Ex2-3	GCACCCCTCACAGGTGATAA	Ex3	102	112	0,9843