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. 2011 Jan 5;6(1):e15832. doi: 10.1371/journal.pone.0015832

Figure 1. Functional organization of pAIM.

Figure 1

The vector is shown in the form of a provirus collinearly integrated in a host gene. The vector is flanked by promoter-deficient LTRs (“dLTR”), which harbor Cre-recombinase recognition sites. The immediate early promoter and enhancer region from human cytomegalovirus (“CMV”) is preceded by a cluster of tet-operators (“TO”), which permit suppression of this promoter in the presence of a tetracycline-controlled transcription silencer (tTS). The coding region of copGFP (“copGFP”) ends with the 2A sequence and an unpaired splice donor (“2A” and “SD” respectively). In the scenario shown above the vector, CMV-driven transcript is polyadenylated on the LTR-derived sequence and encodes only copGFP. In the scenario shown below the vector, transcription continues into the host DNA, and a mature fusion product is produced by splicing, which removes the LTR sequences. If the open reading frames of copGFP and the host gene coincide, both copGFP and a host protein could be produced when the RNA is translated.