Table 6.

CsCl Purified BAC DNA Transgene Integration. Two or More Genotyping Markers for Integration Show Evidence of Fragmented BAC Molecules.

BAC Transgene	Size (kb)	Number of Foundersa	SP6 Assayb	T7 Assayc	Internal Assayd	Expression Positive?e
1417	219	6	+	+	+	yes
		5	+	+	-
		3	+	-	-
1537	76	9	+	+	+	yes
		6	+	-	+
1557	225	3	+	+	+	yes
		2	-	-	+
		2	-	+	-
1598	225	5	+	+	+	yes
1732	240	3	+	+	+	yes
		3	+	+	-
		5	-	-	+
		2	+	-	+
		1	+	-	-
1733	175	5	+	+	+	yes
		2	-	-	+
1652	192	4	n.a.	n.a.	+,+	n.a.
1653	174	9	n.a.	n.a.	+,+	n.a.
		1	n.a.	n.a.	+,-
		1	n.a.	n.a.	-,+
1669	204	3	n.a.	n.a.	+,+	n.a.
		2	n.a.	n.a.	+,-
1928	232	7	+	+	+	yes
		11	+	-	-
		3	-	+	-
		1	-	-	+
		5	+	+	-
1942	232	7	+	+	+	yes
		5	+	+	-
		1	-	+	-
1697	300	3	n.a.	n.a.	+	yes
1698	350	6	n.a.	n.a.	+	yes
1904	200	8	n.a.	n.a.	+	yes

^aNumber of transgene integration positive founder mice.

^bResult of PCR assay to detect SP6 – genomic boundary in BAC transgene.

^cResult of PCR assay to detect T7 – genomic boundary in BAC transgene.

^dResult of PCR assay to detect internal sequences unique to BAC transgene.

^eOutcome of transgene expression analyses.

n.a. Data are not available.