Figure 2. Math model validation: the Neural Colony Forming Cell Assay (N-CFCA).

Cells from each group were cultured in the N-CFCA. The graphs compare in each group, the number of large colonies, greater than 2 mm in diameter (the only size range exhibiting all the key tissue culture stem cell features) obtained after 21 days in vitro. *p = 0.039, **p = 5.38×10⁻¹¹, n = 15, t-test, 2 tails.