Table 1. Symmetric cell division rate.
Groups | 1 | 2 | 3 | |||
E14-EGF + bFGF | 24 months-EGF | E14-EGF | E14-EGF + bFGF | Adult-EGF | Adult- EGF + bFGF | |
F | 22.2 | 3.1 | 13.6 | 18.1 | 8.0 | 12.6 |
Time (days) | 7 | 10 | 7 | 7 | 7 | 7 |
Kll (divisions/day) | 0.44 | 0.11 | 0.37 | 0.41 | 0.30 | 0.36 |
NSCs harvested from ganglionic eminences of E14 mice or from the periventricular region of adult mice were cultured with EGF and bFGF or with EGF alone respectively. The fold expansions (F) were compared, from which the effective rate of stem cell symmetric division (Kll) was calculated using the time for a passage.