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. Author manuscript; available in PMC: 2012 Feb 1.
Published in final edited form as: Proteins. 2011 Feb;79(2):347–351. doi: 10.1002/prot.22906

Table I.

Intracellular Concentrationsa

Protein Expression Level (fg/cell)b


After 1.5 h, leakage After 3 h, leakage Location

HdeA 45 ±4c, no 72 ±7, yes Periplasim
α-Synuclein 21 ±7, no 41 ±9, no Periplasm/Cytoplasm
Chymotrypsin Inhibitor 2 55 ±7, no 100 ±11, yes Cytoplasm/Periplasm
Ubiquitin 12 ±4, no 32 ±6, no Cytoplasm
a

Quantified by integrating pixel intensities of bands from Coomassie-stained SDS PAGE and comparing them against standards of the pure proteins.18

b

Values were obtained from unsaturated cultures, and cannot be used to assess protein yield from saturated cultures.

c

Standard error, n=4.