Table I.
Protein | Expression Level (fg/cell)b | ||||
---|---|---|---|---|---|
After 1.5 h, | leakage | After 3 h, | leakage | Location | |
HdeA | 45 ±4c, | no | 72 ±7, | yes | Periplasim |
α-Synuclein | 21 ±7, | no | 41 ±9, | no | Periplasm/Cytoplasm |
Chymotrypsin Inhibitor 2 | 55 ±7, | no | 100 ±11, | yes | Cytoplasm/Periplasm |
Ubiquitin | 12 ±4, | no | 32 ±6, | no | Cytoplasm |
Quantified by integrating pixel intensities of bands from Coomassie-stained SDS PAGE and comparing them against standards of the pure proteins.18
Values were obtained from unsaturated cultures, and cannot be used to assess protein yield from saturated cultures.
Standard error, n=4.