Fig. 4.

Compound 11 inhibits Hsp27 phosphorylation by MK3 and MK5, but not by MK2. Recombinant Hsp27 was incubated with purified MK2, MK3, or MK5 in the presence or absence of compound 11 . Hsp27 phosphorylation at serine residue 78 (p-Ser78 Hsp27) was monitored by western blot with phosphoserine-78 specific antibodies. Lanes 1, 3, and 5: Hsp27 incubated with purified MK5, MK3, and MK2, respectively. Lanes 2, 4, and 6: as lanes 1, 3, and 5 but in the presence of 20 μM noroxoaconitine (compound 11)