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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Oncogene. 2010 Aug 23;29(49):6418–6427. doi: 10.1038/onc.2010.373

Figure 2. Resistance to Taxol in APC-deficient intestinal crypts is independent of activation of the canonical WNT/beta-catenin pathway.

Figure 2

Mice injected with either vehicle (NT) or 10mg/kg Taxol IP (n≥3) were sacrificed 6h post-injection and the levels of mitotic arrest and apoptosis in intestinal crypts were scored by H+E staining. Mitotic index was expressed as percentage of cells per crypt. (a) IHC detecting beta-catenin showing nuclear localisation in Cre+ APC fl/fl (vehicle or Taxol 10mg/kg treated), Cre+ APC fl/fl MYC fl/fl (10mg/kg Taxol treated) and Cre+ GSK3 alpha fl/fl beta fl/fl mice. Note non-nuclear localisation of beta-catenin in WT vehicle or Taxol 10mg/kg treated intestine. (b) Mitotic index in intestinal crypts from Taxol 10mg/kg or vehicle treated WT, Cre+ MYC fl/fl, Cre+ APC fl/fl, or Cre+ APC fl/fl MYC fl/fl mice. There is no significant difference between the response in Cre+ APC fl/fl and Cre+ APC fl/fl MYC fl/fl mice and between WT and Cre+ MYC fl/fl mice, p>0.05. (c) Representative H+E staining of crypts from Cre+ MYC fl/fl (Taxol 10mg/kg) and Cre+ APC fl/fl MYC fl/fl mice (Taxol 10mg/kg). (d) Mitotic index in intestinal crypts from vehicle or Taxol 10mg/kg treated WT and Cre+ GSK3 alpha fl/fl beta fl/fl mice. (e) Representative H+E staining of crypts from Cre+ APC fl/fl, or Cre+ GSK3 alpha fl/fl beta fl/fl mice treated with Taxol 10mg/kg.