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. Author manuscript; available in PMC: 2011 Dec 1.
Published in final edited form as: Oncogene. 2010 Aug 23;29(49):6418–6427. doi: 10.1038/onc.2010.373

Figure 4. APC is not required as a general mitotic spindle checkpoint protein.

Figure 4

Mice injected with either vehicle (NT), 5 or 10mg/kg Vinorelbine IV (n≥3) were sacrificed 6h post-injection and the levels of mitotic arrest and apoptosis in intestinal crypts were scored by H+E staining. Mitotic and apoptotic index was expressed as percentage of cells per crypt. (a) Mitotic index in intestinal crypts from WT or Cre+ APC fl/fl mice treated with vehicle, 5 or 10mg/kg Vinorelbine. The induction in mitosis is significant for both 5 and 10mg/kg Vinorelbine treated versus untreated p<0.026. (b) Tubulin immunofluorescence (green) was performed on PFA-fixed tissue from WT or Cre+ APC fl/fl mice treated with 10mg/kg Vinorelbine and taken 6h post-injection. Slides were counterstained with DAPI (blue). (c) Representative H+E staining of crypts from WT or Cre+ APC fl/fl mice treated with Vinorelbine 10mg/kg and sacrificed at 3h, 6h or 24h post-injection. NT – vehicle-treated. Scale bar 20μm. (d) Deletion of the bona fide mitotic spindle checkpoint protein Bub1 (Cre+ Bub1 fl/fl mice) leads to complete abrogation of mitotic arrest in intestinal crypts following treatment with either 10, 20mg/kg Taxol or 10mg/kg Vinorelbine.