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. Author manuscript; available in PMC: 2011 Jan 6.
Published in final edited form as: Cell Tissue Res. 2010 Nov 16;342(3):401–410. doi: 10.1007/s00441-010-1077-2

Fig. 5.

Fig. 5

Expression of filaggrin is downregulated in the K14-Cre/caBmprIB epidermis. a, b The granular layer, outlined by dashed lines and indicated by arrows in the control skin (a), was greatly attenuated in P7 K14-Cre/caBmprIB epidermis (b). Note the extremely thin granular layer (arrows in b). The depth of the epidermis is shown by yellow bars. c, d Immunostaining showed profilaggrin/filaggrin expression in the epidermis of P7 wild-type control (c) and transgenic mice (d). Significant downregulation of profilaggrin/filaggrin was observed in the transgenic epidermis (arrows granular layer, dashed lines boundary of basal layer). e, f Loricrin expression was slightly downregulated in the epidermis of a P7 transgenic skin sample (f), as compared with its expression in the wild-type control (e). g Real-time RT-PCR demonstrated decreased profilaggrin mRNA expression in the epidermis of P1 and P7 K14-Cre/caBmprIB (Tg) mice, as compared with wild-types (Wt). h Profilaggrin and filaggrin (36 kDa) expression was equally decreased in transgenic mice. K10 expression was used as a loading control. Bars 50 µm (a–d), 80 µm (e, f)