. Author manuscript; available in PMC: 2011 Jan 6.

Published in final edited form as: Gene Ther. 2009 Sep 17;17(1):50–60. doi: 10.1038/gt.2009.123

Table 1.

Rhesus erythropoietin (RhEpo) levels in aqueous extracts of macaque parotid glands

Animal number →	AAV2RhEpo			AAV5RhEpo
Animal number →	103	3559	4810	4727	4731	4798
RhEpo (mU mg⁻¹ protein)	26.9	37.6	20.2	0.7	0.8	1.7

Thirty–50 mg of parotid gland tissue from AAV5 (herein) and AAV2⁸-vector transduced macaques were suspended in Cell Culture Lysis Reagent (Promega) and homogenized with an Omni TH 1 homogenizer to extract protein. Fifty μl of this extract were used to determine the presence of RhEpo protein with a human erythropoietin ELISA kit (StemCell Technologies, Vancouver, BC, Canada). The concentration of protein was determined using the BCA protein assay (Pierce, Rockford, Il, USA). Each gland sample, indicated by animal number and the vector administered, was tested in duplicate and average values are shown.