. Author manuscript; available in PMC: 2011 Jan 6.

Published in final edited form as: Gene Ther. 2009 Sep 17;17(1):50–60. doi: 10.1038/gt.2009.123

Table 4.

IFN-γ production detected by ELISPOT after incubation of rhesus PMBCs with autologous fibroblasts

Animal number	Treatment	Spots per well	Experiment –control
4727	PBMC alone	0	—
	PBMC+control fibroblasts	4 ± 3	4
	PBMC+autologous fibroblasts	3 ± 3	3
	PBMC+ConA	104 ± 18	104
4731	PBMC alone	0	—
	PBMC+control fibroblasts	2 ± 2	2
	PBMC+autologous fibroblasts	5 ± 2	5
	PBMC+ConA	148 ± 16	148

Abbreviations: Con A, concanavalin A; CTL, cytotoxic lymphocyte; IFNγ, interferon-γ; MOI, multiplicity of infection; PBMC, peripheral blood mononuclear cells; PVDF, polyvinylidene difluoride.

The data shown are mean values ( ± s.d.) for the results of ELISPOT assays for IFNγ secretion after incubation of PBMCs isolated from two high-dose monkeys (4727, 4731) with target cells (uninfected or AAV5-transduced autologous fibroblasts). Fibroblasts were transduced or not with ~5000 MOI of AAV5 vector encoding a green fluorescence protein. The number of AAV5-specific CTLs was determined by counting the IFNγ-positive spots per PVDF membrane.