Table 4.
Animal number | Treatment | Spots per well | Experiment –control |
---|---|---|---|
4727 | PBMC alone | 0 | — |
PBMC+control fibroblasts | 4 ± 3 | 4 | |
PBMC+autologous fibroblasts | 3 ± 3 | 3 | |
PBMC+ConA | 104 ± 18 | 104 | |
4731 | PBMC alone | 0 | — |
PBMC+control fibroblasts | 2 ± 2 | 2 | |
PBMC+autologous fibroblasts | 5 ± 2 | 5 | |
PBMC+ConA | 148 ± 16 | 148 |
Abbreviations: Con A, concanavalin A; CTL, cytotoxic lymphocyte; IFNγ, interferon-γ; MOI, multiplicity of infection; PBMC, peripheral blood mononuclear cells; PVDF, polyvinylidene difluoride.
The data shown are mean values ( ± s.d.) for the results of ELISPOT assays for IFNγ secretion after incubation of PBMCs isolated from two high-dose monkeys (4727, 4731) with target cells (uninfected or AAV5-transduced autologous fibroblasts). Fibroblasts were transduced or not with ~5000 MOI of AAV5 vector encoding a green fluorescence protein. The number of AAV5-specific CTLs was determined by counting the IFNγ-positive spots per PVDF membrane.