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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: J Immunol. 2010 Jun 11;185(2):1265–1273. doi: 10.4049/jimmunol.0902808

FIGURE 1.

FIGURE 1

Particle phagocytosis inhibits RANKL-mediated osteoclastogenesis. Preosteoclasts were cultured for 9 d in the presence of 25 ng/ml M-CSF in the presence or absence of RANKL (RL; 50 ng/ml). Particles of PMMA or titanium (Ti; 20 particles/cell) or IFN-γ (1.5 or 10 U/ml) were added at the same time as the RANKL. Following culture, cells were either stained for TRAP or processed for RNA extraction and qPCR. A, Representative images of TRAP-stained cells (original magnification ×50) and quantitation of TRAP-positive multinuclear (≥3 nuclei) cells. n = 3. B, Dose dependency of particle-mediated inhibition of RANKL-mediated osteoclastogenesis. n = 3. C, qPCR analysis of cathepsin K and Annexin VIII mRNA expression relative to GAPDH. n = 4. *p < 0.01 compared with RANKL alone.