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. Author manuscript; available in PMC: 2011 Jul 15.
Published in final edited form as: J Immunol. 2010 Jun 11;185(2):1265–1273. doi: 10.4049/jimmunol.0902808

FIGURE 2.

FIGURE 2

RANKL pretreatment blunts particle phagocytosis-mediated inhibition of osteoclastogenesis. Preosteoclasts were cultured for 9 d in the presence of 25 ng/ml M-CSF and 50 ng/ml RANKL. Particles of PMMA or titanium (Ti; 20 particles/cell) or IFN-γ (1.5 U/ml) were added at the same time as the RANKL (day 0) or at various times after initial addition of RANKL. A, Representative TRAP staining of cells pretreated with RANKL for 0, 1, and 3 d and quantitation of TRAP-positive multinuclear cells (original magnification ×50). n = 3. B, qPCR for detection of cathepsin K and Annexin VIII mRNA of cells pretreated with RANKL for 0 and 3 d. n = 4. *p < 0.01 compared with RANKL alone.