Figure 3.

Skp2 cooperates with N-Ras to induce T cell lymphomas in mice. (A) Percentage of survival in progeny of transgenic crosses of CD4-SKP2 mice (lines 2 and 16) and TMTV-Nras mice (line A). Nontransgenic control mice and single transgenic mice are indicated as Ctr, Skp2, and N-Ras, respectively; double transgenic mice are indicated as Skp2 + N-Ras. The percentage of survivors is given against the age in days. No significant difference in survival was observed when lines 2 and 16 were analyzed independently (not shown). (B) Immunoblotting of tissue extracts (30 μg of protein) from control thymi (Ctr) and tumors developed in TMTV-Nras mice (R) or in double transgenic mice (S + R). Numbers represent different animals from which tissues were obtained. Extracts were immunoblotted with the antibodies to the indicated proteins. The asterisk in the E2F-1 panel indicates a nonspecific band.