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. 2010 Nov 15;20(3):510–527. doi: 10.1093/hmg/ddq496

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© The Author 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Representative western blots of GST-APs for proteins that interacted with the CACNA1A_MPI but not CACNA1A_MPc isoform by Y2H mating assay (Table 1). The myc-tagged prey and GST-tagged baits are indicated (empty vector, MPc or MPI polyQ length). Blotting antibodies are anti-myc and anti-GST as indicated. (A) The myc-TSC22D1 interaction is neither isoform nor polyQ length dependant in mammalian cells. (B) The myc-RBM12B interaction with CACNA1A is not isoform specific, but for MPI is polyQ length dependant, interacting with the disease-causing 23Q and hyper-expanded 72Q but not the wild-type 11Q baits. (C) The myc-BZRAP1 interaction with CACNA1A is isoform, but not polyQ length dependant. (D) The myc-YLPM1 interaction with CACNA1A is both isoform and polyQ length dependant.